Reaction-based near-infrared fluorescent probes for monitoring NADH-associated redox remodeling in ferroptosis
Document Type
Article
Publication Date
10-15-2026
Abstract
Nicotinamide adenine dinucleotide (NADH) plays a central role in cellular redox balance and metabolic regulation, yet its dynamic behavior during ferroptosis remains insufficiently understood. Herein, we report reaction-based fluorescent probes, QX-I and QX-II , for imaging NADH-associated redox changes. Two probes were constructed by conjugating a responsive group (methyl-quinolinium salt) with an electron-withdrawing Xanthene moiety. The conjugated Xanthene structure not only shifts the emission wavelength of the probes into the near-infrared region, but also endows probe QX-I (modified with a single N-ethyl group) with advantages such as high fluorescence enhancement, rapid response, high sensitivity, and good selectivity. This probe enables visualization of endogenous NADH fluctuations in HepG2 and 4 T1 cells under metabolic regulation. Notably, QX-I was further employed to investigate NADH-related dynamic changes during ferroptosis. Cell and in vivo experiments demonstrated a decoupling relationship between lipid peroxidation and NADH-related redox states during ferroptosis inhibition. Overall, this study provides an effective tool for monitoring NADH-related redox remodeling and offers new insights into ferroptosis-associated redox metabolism.
Publication Title
Bioorganic Chemistry
Recommended Citation
Li, Y.,
Sha, Y.,
He, D.,
Yu, Y.,
You, Y.,
Ye, T.,
Liu, H.,
Wang, J.,
Li, J.,
&
Fang, M.
(2026).
Reaction-based near-infrared fluorescent probes for monitoring NADH-associated redox remodeling in ferroptosis.
Bioorganic Chemistry,
182.
http://doi.org/10.1016/j.bioorg.2026.110493
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p2/2963