Document Type

Article

Publication Date

4-18-2025

Department

Department of Biomedical Engineering

Abstract

Biomaterials with inherent anti-inflammatory properties and the ability to foster a pro-regenerative environment hold significant promise for enhancing cell transplantation and tissue regeneration. Fucoidan, a sulfated polysaccharide with well-documented immune-regulatory and antioxidant capabilities, offers strong potential for creating such biomaterials. Yet, there is a lack of engineered fucoidan hydrogels that are injectable and provide tunable physicochemical properties. In this study, the ability of fucoidan to undergo periodate-mediated oxidation is leveraged to introduce aldehydes into backbone (oxidized fucoidan, OFu), enabling the formation of reversible, imine-crosslinks with amine-containing molecules such as gelatin. The imine-crosslinked OFu-gelatin hydrogel provided excellent control over gelation rate and mechanical properties. Counter-intuitively, OFu-gelatin hydrogel exhibited excellent long-term stability (≥28 days), even though imine crosslinks are known to be relatively less stable. Moreover, the OFu-gelatin hydrogels are self-healing, injectable, and biocompatible, supporting cell culture and encapsulation. Furthermore, fucoidan hydrogels displayed immune-modulatory properties both in vitro and in vivo. This innovative injectable fucoidan hydrogel presents a versatile platform for applications in tissue engineering and regenerative medicine.

Publisher's Statement

© 2025 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH. Publisher’s version of record: https://doi.org/10.1002/adhm.202405260

Publication Title

Advanced healthcare materials

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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