Arctic Micromonas uses protein pools and non-photochemical quenching to cope with temperature restrictions on Photosystem II protein turnover

Authors

Guangyan Ni, Mount Allison University
Gabrielle Zimbalatti, Mount Allison University
Cole D. Murphy, Mount Allison University
Audrey B. Barnett, Michigan Technological UniversityFollow
Christopher M. Arsenault, Mount Allison University
Gang Li, Mount Allison University
Amanda M. Cockshutt, Mount Allison University
Douglas A. Campbell, Mount Allison University

Document Type

Article

Publication Date

2-2017

Department

Department of Geological and Mining Engineering and Sciences

Abstract

Micromonas strains of small prasinophyte green algae are found throughout the world’s oceans, exploiting widely different niches. We grew arctic and temperate strains of Micromonas and compared their susceptibilities to photoinactivation of Photosystem II, their counteracting Photosystem II repair capacities, their Photosystem II content, and their induction and relaxation of non-photochemical quenching. In the arctic strain Micromonas NCMA 2099, the cellular content of active Photosystem II represents only about 50 % of total Photosystem II protein, as a slow rate constant for clearance of PsbA protein limits instantaneous repair. In contrast, the temperate strain NCMA 1646 shows a faster clearance of PsbA protein which allows it to maintain active Photosystem II content equivalent to total Photosystem II protein. Under growth at 2 °C, the arctic Micromonas maintains a constitutive induction of xanthophyll deepoxidation, shown by second-derivative whole-cell spectra, which supports strong induction of non-photochemical quenching under low to moderate light, even if xanthophyll cycling is blocked. This non-photochemical quenching, however, relaxes during subsequent darkness with kinetics nearly comparable to the temperate Micromonas NCMA 1646, thereby limiting the opportunity cost of sustained downregulation of PSII function after a decrease in light.

Publisher's Statement

© 2016, The Author(s). Publisher’s version of record: https://doi.org/10.1007/s11120-016-0310-6

Correction: https://doi.org/10.1007/s11120-017-0471-y

Publication Title

Photosynthesis Research

Recommended Citation

Ni, G., Zimbalatti, G., Murphy, C., Barnett, A., Arsenault, C., Li, G., Cockshutt, A., & Campbell, D. (2017). Arctic Micromonas uses protein pools and non-photochemical quenching to cope with temperature restrictions on Photosystem II protein turnover. Photosynthesis Research, 131(2), 203-220. http://doi.org/10.1007/s11120-016-0310-6
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/5007

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF