Document Type
Article
Publication Date
4-20-2023
Department
Department of Biological Sciences
Abstract
Grain weight and quality are always determined by the grain filling. Plant miRNAs have drawn attention as key targets for regulating grain size and yield. Yet the mechanisms underlying the regulation of grain size are largely unclear due to the complex networks controlling this trait. Our earlier studies proved that the suppressed expression of miR167 (STTM/MIM167) substantially increased grain weight. In a field test, the increased yield up to 12.90%-21.94% due to the significantly enhanced grain filling rate. Biochemical and genetic analyses reveal the regulatory effects of miR159 on miR167 expression. Further analysis indicates that OsARF12 is the major mediator of miR167 in regulating rice grain filling. Expectedly, over expressing OsARF12 could resemble the phenotype of STTM/MIM167 plants with respect to grain weight and grain filling rate. Upon in-depth analysis, we found that OsARF12 activates OsCDKF;2 expressions by directly binding to the TGTCGG motif in the promoter region. Flow cytometric analysis in young panicles of plants overexpressing OsARF12 and cell number examination of cdkf;2 mutants verify that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2. Moreover, RNA-seq result suggests that miR167-OsARF12 module is involved in the cell development process and hormone pathways. Additionally, plants overexpressing OsARF12 or cdkf;2 mutants present enhanced or reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatments, confirming that OsCDKF;2 targeting by OsARF12 mediates auxin and BR signaling. Our results reveal that miR167-OsARF12 module works downstream of miR159 to regulate rice grain filling and grain size by OsCDKF;2 through controlling cell division and mediating auxin and BR signals.
Publication Title
Plant communications
Recommended Citation
Zhao, Y.,
Zhang, X.,
Cheng, Y.,
Du, X.,
Teotia, S.,
Miao, C.,
Sun, H.,
Fan, G.,
Tang, G.,
Xue, H.,
Zhao, Q.,
&
Peng, T.
(2023).
The miR167-OsARF12 module regulates grain filling and grain size downstream of miR159.
Plant communications, 100604.
http://doi.org/10.1016/j.xplc.2023.100604
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/17095
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Version
Publisher's PDF
Publisher's Statement
© 2023 The Author(s). Publisher’s version of record: https://doi.org/10.1016/j.xplc.2023.100604