Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
Document Type
Article
Publication Date
7-7-2022
Department
Department of Chemistry
Abstract
Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD). Here, we describe a method for direct determination of OD of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.
Publication Title
PeerJ analytical chemistry
Recommended Citation
Chillar, K.,
Yin, Y.,
Eriyagama, A. M.,
&
Fang, S.
(2022).
Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area.
PeerJ analytical chemistry,
4.
http://doi.org/10.7717/peerj-achem.20
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/16258