Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells

Document Type

Article

Publication Date

1-1-2022

Department

Department of Chemistry

Abstract

A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.

Publication Title

Methods

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