Response of endothelial cells to decellularized extracellular matrix deposited by bone marrow mesenchymal stem cells.
Document Type
Article
Publication Date
1-1-2014
Department
Department of Biomedical Engineering
Abstract
OBJECTIVE: Evaluate the behavior and function of human umbilical vein endothelial cells (HUVECs) on decellularized extracellular matrix (ECM) deposited by bone marrow mesenchymal stem cells (BMSCs).
METHODS: Prepared through chemical approach, decellularized ECM was characterized by use of immunofluorescence staining. The morphology, attachment, proliferation and migration of HUVECs cultured on six-well tissue culture plastic (TCP) and decellularized ECM were investigated.
RESULTS: Decellularized ECM was successfully prepared without three-dimensional architecture disruption. This biological scaffold is similar to nature vascular ECM, preserved various matrix proteins such as type I collagen, type III collagen and fibronection. HUVECs on decellularized ECM showed well attachment and regular arrangement. Decellularized ECM could also significantly enhance the migration and proliferation potential of HUVECs in contrast to TCP.
CONCLUSION: Deposited by BMSCs, ECM can affect the behavior of endothelial cell and could be used as a promising material in tissue engineering.
Publication Title
International Journal of Clinical and Experimental Medicine
Recommended Citation
Xu, Y.,
Yan, M.,
Gong, Y.,
Chen, L.,
Zhao, F.,
&
Zhang, Z.
(2014).
Response of endothelial cells to decellularized extracellular matrix deposited by bone marrow mesenchymal stem cells..
International Journal of Clinical and Experimental Medicine,
7(12), 4997-5003.
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/1289