Cloning of protocatechuate 3,4-dioxygenase genes from Bradyrhizobium japonicum USDA110

Document Type

Article

Publication Date

1-1-1993

Abstract

A heterologous gene probe encoding the α and β subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.

Publication Title

Applied and Environmental Microbiology

Share

COinS