Cloning of protocatechuate 3,4-dioxygenase genes from Bradyrhizobium japonicum USDA110
Document Type
Article
Publication Date
1-1-1993
Abstract
A heterologous gene probe encoding the α and β subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.
Publication Title
Applied and Environmental Microbiology
Recommended Citation
Podila, G.,
Kotagiri, S.,
&
Shantharam, S.
(1993).
Cloning of protocatechuate 3,4-dioxygenase genes from Bradyrhizobium japonicum USDA110.
Applied and Environmental Microbiology,
59(8), 2717-2719.
http://doi.org/10.1128/aem.59.8.2717-2719.1993
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/12263