Functional characterization of CCR in birch (Betula platyphylla × Betula pendula) through overexpression and suppression analysis
Document Type
Article
Publication Date
1-1-2015
Abstract
© 2014 Scandinavian Plant Physiology Society. We cloned a Cinnamoyl-CoA Reductase gene (BpCCR1) from an apical meristem and first internode of Betula platyphylla and characterized its functions in lignin biosynthesis, wood formation and tree growth through transgenic approaches. We generated overexpression and suppression transgenic lines and analyzed them in comparison with the wild-type in terms of lignin content, anatomical characteristics, height and biomass. We found that BpCCR1 overexpression could increase lignin content up to 14.6%, and its underexpression decreased lignin content by 6.3%. Surprisingly, modification of BpCCR1 expression led to conspicuous changes in wood characteristics, including xylem vessel number and arrangement, and secondary wall thickness. The growth of transgenic trees in terms of height was also significantly influenced by the modification of BpCCR1 genes. We discuss the functions of BpCCR1 in the context of a phylogenetic tree built with CCR genes from multiple species.
Publication Title
Physiologia Plantarum
Recommended Citation
Zhang, W.,
Wei, R.,
Chen, S.,
Jiang, J.,
Li, H.,
Huang, H.,
Yang, G.,
Wang, S.,
Wei, H.,
&
Liu, G.
(2015).
Functional characterization of CCR in birch (Betula platyphylla × Betula pendula) through overexpression and suppression analysis.
Physiologia Plantarum,
154(2), 283-296.
http://doi.org/10.1111/ppl.12306
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/11497