Novel phosphoinositides in barley aleurone cells: Additional evidence for the presence of phosphatidyl-scyllo-inositol
Document Type
Article
Publication Date
1-1-1997
Abstract
A novel isomer of phosphatidylinositol that differs in the structure of the head group was detected in barley (Hordeurn vulgate cv Himalaya) seeds. In this paper we describe our efforts to elucidate the structure of the novel isomer. Evidence from a variety of techniques, including chemical modification of in vivo 32Pi- and myo-[3H]inositol-labeled compounds, gas chromatography-mass spectrometry analysis, in vivo incorporation of scyllo-[3H]inositol, and enzymatic studies that suggest that the structure is phosphatidylscyllo-inositol (scyllo-PI), is presented. The use of microwave energy to significantly enhance the slow rate of hydrolysis of phosphoinositides is described. The presence of scyllo-PI can be easily overlooked by the methods commonly employed; therefore, experimental considerations important for the detection of scyllo-PI are discussed.
Publication Title
Plant Physiology
Recommended Citation
Narasimhan, B.,
Pliska-Matyshak, G.,
Kinnard, R.,
Carstensen, S.,
Ritter, M.,
Von Weymarn, L.,
&
Murthy, P.
(1997).
Novel phosphoinositides in barley aleurone cells: Additional evidence for the presence of phosphatidyl-scyllo-inositol.
Plant Physiology,
113(4), 1385-1393.
http://doi.org/10.1104/pp.113.4.1385
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/10134