Date of Award

2017

Document Type

Open Access Master's Thesis

Degree Name

Master of Science in Biomedical Engineering (MS)

Administrative Home Department

Department of Biomedical Engineering

Advisor 1

Megan Frost

Committee Member 1

Martin Thompson

Committee Member 2

Smitha Rao

Abstract

Nitric oxide (NO) has been shown to possess many Anti-inflammatory effects. Ascorbic acid which is naturally present in human body is known to react with S-nitrosothiols (RSNO) directly to reduce RSNO to NO and produce free thiol groups, if present in more than millimolar concentration. Higher concentration of ascorbic acid is required because the reduction of RSNO is not caused by ascorbate instead, but instead a decomposed product of ascorbic acid. S-Nitroso-N-Acetylpenicillamine is used as an NO donor. An HPLC method is used for determination of the degradation product of ascorbic acid and simultaneously identifying the compound responsible for NO production from RSNO by running the compound through Nitric Oxide Analyzer. Once the compound is identified it has a potential medical use for NO production. For example, it could provide a means that initiates RSNO decomposition in polymeric biomaterials. Thus, increasing the functionality of the medical device coated with such materials and preventing thrombus formation.

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