Date of Award

2025

Document Type

Open Access Master's Thesis

Degree Name

Master of Science in Chemistry (MS)

Administrative Home Department

Department of Chemistry

Advisor 1

Haiying Liu

Committee Member 1

Lanrong Bi

Committee Member 2

Tarun Dam

Committee Member 3

Momoko Tajiri

Abstract

Two near-infrared fluorescent sensors, sensor A and sensor B, were synthesized and characterized. Sensor A was a hemicyanine derived structure, and sensor B was a BODIPY derived sensor. The sensors were tested to measure their ability to monitor viscosity, and sensor A was used to monitor viscosity in live cells. Both sensors were found to be effective in monitoring the viscosity level through optical measurements with glycerol. Sensor A was found to be successful in monitoring mitochondrial viscosity in HeLa MBA-MD-453 and MDA-MB-231 cell lines. It was also successful in monitoring viscosity in Drosophila melanogaster larvae as well as in ADPKD affected mice kidney tissue. In all of these scenarios, the fluorescent intensity increased with viscosity. Sensor A has provided significant evidence that it would be a useful tool in monitoring mitochondrial viscosity in a variety of cellular conditions.

Available for download on Tuesday, October 14, 2025

Included in

Biochemistry Commons

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