Date of Award
2025
Document Type
Open Access Master's Thesis
Degree Name
Master of Science in Chemistry (MS)
Administrative Home Department
Department of Chemistry
Advisor 1
Haiying Liu
Committee Member 1
Lanrong Bi
Committee Member 2
Tarun Dam
Committee Member 3
Momoko Tajiri
Abstract
Two near-infrared fluorescent sensors, sensor A and sensor B, were synthesized and characterized. Sensor A was a hemicyanine derived structure, and sensor B was a BODIPY derived sensor. The sensors were tested to measure their ability to monitor viscosity, and sensor A was used to monitor viscosity in live cells. Both sensors were found to be effective in monitoring the viscosity level through optical measurements with glycerol. Sensor A was found to be successful in monitoring mitochondrial viscosity in HeLa MBA-MD-453 and MDA-MB-231 cell lines. It was also successful in monitoring viscosity in Drosophila melanogaster larvae as well as in ADPKD affected mice kidney tissue. In all of these scenarios, the fluorescent intensity increased with viscosity. Sensor A has provided significant evidence that it would be a useful tool in monitoring mitochondrial viscosity in a variety of cellular conditions.
Recommended Citation
Peters, Joseph, "NEAR-INFRARED HEMICYANINE AND BODIPY SENSORS FOR MONITORING MITOCHONDRIAL VISCOSITY IN LIVE CANCER CELLS, DROSOPHILA MELANOGASTER LARVAE, AND ADPKD KIDNEY TISSUE", Open Access Master's Thesis, Michigan Technological University, 2025.