Date of Award
2024
Document Type
Open Access Master's Report
Degree Name
Master of Science in Chemical Engineering (MS)
Administrative Home Department
Department of Chemical Engineering
Advisor 1
Adrienne Minerick
Committee Member 1
Timothy Eisele
Committee Member 2
Robert Handler
Abstract
Diabetes affects 537 million people worldwide, while 136 million Americans have diabetes or prediabetes; roughly 22.6% of individuals are undiagnosed. Diabetes is the inability to regulate blood sugar levels. Hemoglobin A1c (HbA1c) is glycosylated hemoglobin and indicates average blood sugar levels over the past three months.
We adapted the selective boronic acid binding to the HbA1c sugar moieties from boronate affinity chromatography and translated it into a portable microchip paper-based electrophoresis platform. We explored a range of boronate concentrations at a steady voltage and sample volume to determine the optimal HbA1c binding and subsequent electrophoretic separation. A pH above 11 was determined optimal to overcome the blood sample's natural buffering capacity and favor binding to HbA1c.
Video images of the band separation were acquired but will be analyzed further using image processing software to quantify bandwidths for HbA1c level quantification and diabetic status determination.
Recommended Citation
Fozilova, Rixlie, "OPTIMIZING DIABETES DIAGNOSTICS: ELECTROPHORETIC SEPARATION AND DETECTION OF HEMOGLOBIN A1C WITH BORONATE", Open Access Master's Report, Michigan Technological University, 2024.