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Date of Award


Document Type

Campus Access Master's Report

Degree Name

Master of Science in Chemistry (MS)

Administrative Home Department

Department of Chemistry

Advisor 1

Shiyue Fang

Committee Member 1

Lanrong Bi

Committee Member 2

Marina Tanasova


Chemical synthesis of long oligo deoxynucleotides via the catching-by-polymerization method has been used to address the various drawbacks encountered during traditional polymerase chain reaction assembly. However, the catching-by-polymerization method can encounter errors during synthesis. One of those errors, called substitution error, has been observed during the capping step of failure sequences after addition of deoxyguanosine nucleotides to the ODN strand. This report discusses a synthesis scheme during deoxyguanosine preparation to chlorinate the N2 carbon. This can prevent unintended nucleophilic attack during capping, which may result in the observed substitution error during final deprotection of synthesized oligo deoxynucleotides.