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Date of Award
2024
Document Type
Campus Access Master's Thesis
Degree Name
Master of Science in Biological Sciences (MS)
Administrative Home Department
Department of Biological Sciences
Advisor 1
Paul Goetsch
Committee Member 1
Gordon Paterson
Committee Member 2
Guiliang Tang
Abstract
Transgenic reporters are ubiquitous in the field of genetics, and new reporter systems hold promise to drastically increase throughput of data collection. The use of fluorescent, bioluminescent and pigment reporters have emerged as a means of studying gene expression in diverse genetic model systems. To date, fluorescent reporters are the most common system used to study gene expression in Caenorhabditis elegans, but fluorescence has its drawbacks. The aim of this project was to design and implement alternative reporter systems for studying and quantifying gene expression in vivo in the model organism C elegans to increase data generation throughput and ease of use. This project outlines the design of a firefly luciferase (FLUC) and Nano-luciferase (NLUC) dual reporter system along with a betanin pigmentation reporter system (RUBY) adapted for C. elegans use. This project also establishes a reproducible method for in vivo quantification of luminescence from the FLUC reporter.
Recommended Citation
Norris, Dylan D., "DESIGN AND APPLICATION OF ALTERNATIVE TRANSGENIC REPORTER SYSTEMS IN CAENORHABDITIS ELEGANS", Campus Access Master's Thesis, Michigan Technological University, 2024.