Date of Award

2020

Document Type

Open Access Master's Report

Degree Name

Master of Science in Chemical Engineering (MS)

Administrative Home Department

Department of Chemical Engineering

Advisor 1

Caryn Heldt

Committee Member 1

Rebecca Ong

Committee Member 2

Timothy Eisele

Abstract

Influenza virus has an intrinsic nature to undergo mutations. Hence, there have been struggles to combat this disease since the time when the worst influenza pandemic first hit in 1918. Vaccines have proved to be effective in controlling the spread of the influenza A virus by providing herd immunity. However, the contemporary design of influenza A virus based on egg and cell-culture is not efficient in tackling influenza A virus transformation. Virus-like particles (VLPs) have established themselves as a potental platform for future vaccine candidates. VLPs have all the credentials to supplant contemporary vaccine designs without compromising on immunogenicity. For expression of VLPs, yeast has all the definitive potential to develop new-generation influenza A vaccines. This report describes how to use Hansenula polymorpha, a yeast, to make VLPs of influenza H1N1 virus.

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