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Date of Award

2013

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Chemistry (PhD)

College, School or Department Name

Department of Chemistry

First Advisor

Lanrong Bi

Abstract

Fluorescent probes are important research tools for real-time imaging of live cells and a powerful tool for cell biologists. The primary goal of my research project is to develop novel fluorescent probes for facilitating biomedical research. During my Ph.D. study, I have designed, synthesized and evaluated a series of novel fluorescent probes. These novel molecular probes provide high sensitivity and great versatility while minimally perturbing the cell under investigation. For example, I have developed a series of novel targetable and trappable fluorescent probes for imaging organelle location, monitoring oxidative stress, sensing small signaling molecules, and DNA damage. Owing to their small size and greater tenability, these novel molecular probes can offer a precise and powerful approach to study biology at the subcellular level with minimal perturbation to the native system compared to traditional biological methods. Application of these new fluorescent probes to cancer, ischemia/reperfusion injury and neurodegenerative diseases will be discussed.

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