Chapter 28 - Virus removal in bioprocessing using charged media
Document Type
Book Chapter
Publication Date
2024
Department
Department of Chemical Engineering; Health Research Institute
Abstract
Viral removal has long been a key part of FDA-approved processes. Anion-exchange chromatography is commonly used to bind negatively charged viruses and separate them from therapeutic products. Each individual process must confirm that virus has been removed. This empirical determination of virus removal is now being pushed toward a more mechanistic understanding of virus removal. There are two key parts of a virus removal operation with charged media: the physicochemical attributes of the virus and proteins in solution, and the chromatography media. With new biophysical and computational tools, improvements are being made to provide a scientific basis for deviations in virus binding. New formats of ion exchange media are also improving the scale-up and reliability of virus removal mechanisms. Virus removal is becoming less of an empirical art and more of a science that will greatly improve the safety of biotherapeutics in the future.
Publication Title
Ion-Exchange Chromatography and Related Techniques
Recommended Citation
Heldt, C.
(2024).
Chapter 28 - Virus removal in bioprocessing using charged media.
Ion-Exchange Chromatography and Related Techniques, 579-590.
http://doi.org/10.1016/B978-0-443-15369-3.00010-9
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p2/2469