Conductive 3D nano-biohybrid systems based on densified carbon nanotube forests and living cells

Authors

Roya Bagheri, Michigan Technological UniversityFollow
Alicia K. Ball, Michigan Technological University
Masoud Kasraie, Michigan Technological UniversityFollow
Aparna Chandra, Michigan Technological University
Xinqian Chen, Michigan Technological UniversityFollow
Ibrahim Miskioglu, Michigan Technological UniversityFollow
Zhiying Shan, Michigan Technological UniversityFollow
Parisa Pour Shahid Saeed Abadi, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

1-1-2023

Department

Department of Mechanical Engineering-Engineering Mechanics; Department of Chemical Engineering; Department of Materials Science and Engineering; Department of Kinesiology and Integrative Physiology; Health Research Institute; Department of Biomedical Engineering

Abstract

Conductive biohybrid cell-material systems have applications in bioelectronics and biorobotics. To date, conductive scaffolds are limited to those with low electrical conductivity or 2D sheets. Here, 3D biohybrid conductive systems are developed using fibroblasts or cardiomyocytes integrated with carbon nanotube (CNT) forests that are densified due to interactions with a gelatin coating. CNT forest scaffolds with a height range of 120–240 µm and an average electrical conductivity of 0.6 S/cm are developed and shown to be cytocompatible as evidenced from greater than 89% viability measured by live-dead assay on both cells on day 1. The cells spread on top and along the height of the CNT forest scaffolds. Finally, the scaffolds have no adverse effects on the expression of genes related to cardiomyocyte maturation and functionality, or fibroblast migration, adhesion, and spreading. The results show that the scaffold could be used in applications ranging from organ-on-a-chip systems to muscle actuators. Graphical abstract: [Figure not available: see fulltext.]

Publisher's Statement

© The Author(s) 2023. Publisher’s version of record: https://doi.org/10.1557/s43578-023-01163-x

Publication Title

Journal of Materials Research

Recommended Citation

Bagheri, R., Ball, A., Kasraie, M., Chandra, A., Chen, X., Miskioglu, I., Shan, Z., & Abadi, P. S. (2023). Conductive 3D nano-biohybrid systems based on densified carbon nanotube forests and living cells. Journal of Materials Research. http://doi.org/10.1557/s43578-023-01163-x
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p2/213

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF