Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated staphylococcus aureus

Authors

Peter K. Lee, University of Minnesota Medical School
Barry N. Kreiswirth, University of Minnesota Medical School
James R. Deringer, University of Minnesota Medical School
Steven J. Projan, University of Minnesota Medical School
William Eisner, University of Minnesota Medical School
Beverly L. Smith, University of Minnesota Medical School
Eunice Carlson, University of Minnesota Medical School
Richard P. Novick, University of Minnesota Medical School
Patrick M. Schlievert, University of Minnesota Medical School

Document Type

Article

Publication Date

1-1-1992

Abstract

Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pi) of 8.5, whereas TSST-bovine has the same pi (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1. © 1992 The University of Chicago.

Publication Title

Journal of Infectious Diseases

Recommended Citation

Lee, P., Kreiswirth, B., Deringer, J., Projan, S., Eisner, W., Smith, B., Carlson, E., Novick, R., & Schlievert, P. (1992). Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated staphylococcus aureus. Journal of Infectious Diseases, 165(6), 1056-1063. http://doi.org/10.1093/infdis/165.6.1056
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/9802