Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated staphylococcus aureus
Document Type
Article
Publication Date
1-1-1992
Abstract
Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pi) of 8.5, whereas TSST-bovine has the same pi (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1. © 1992 The University of Chicago.
Publication Title
Journal of Infectious Diseases
Recommended Citation
Lee, P.,
Kreiswirth, B.,
Deringer, J.,
Projan, S.,
Eisner, W.,
Smith, B.,
Carlson, E.,
Novick, R.,
&
Schlievert, P.
(1992).
Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated staphylococcus aureus.
Journal of Infectious Diseases,
165(6), 1056-1063.
http://doi.org/10.1093/infdis/165.6.1056
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/9802