Document Type
Article
Publication Date
4-1-2018
Department
Department of Mechanical Engineering-Engineering Mechanics
Abstract
In situ liquid cell transmission electron microscopy (LC-TEM) allows dynamic nanoscale characterization of systems in a hydrated state. Although powerful, this technique remains impaired by issues of repeatability that limit experimental fidelity and hinder the identification and control of some variables underlying observed dynamics. We detail new LC-TEM devices that improve experimental reproducibility by expanding available imaging area and providing a platform for investigating electron flux history on the sample. Irradiation history is an important factor influencing LC-TEM results that has, to this point, been largely qualitatively and not quantitatively described. We use these devices to highlight the role of cumulative electron flux history on samples from both nanoparticle growth and biological imaging experiments and demonstrate capture of time zero, low-dose images on beam-sensitive samples. In particular, the ability to capture pristine images of biological samples, where the acquired image is the first time that the cell experiences significant electron flux, allowed us to determine that nanoparticle movement compared to the cell membrane was a function of cell damage and therefore an artifact rather than visualizing cell dynamics in action. These results highlight just a subset of the new science that is accessible with LC-TEM through the new multiwindow devices with patterned focusing aides.
Publication Title
Sci Adv
Recommended Citation
Moser, T.,
Mehta, H.,
Park, C.,
Kelly, R.,
Shokuhfar, T.,
&
Evans, J.
(2018).
The role of electron irradiation history in liquid cell transmission electron microscopy..
Sci Adv,
4(4), 1202-1202.
http://doi.org/10.1126/sciadv.aaq1202
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/973
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Version
Publisher's PDF
Publisher's Statement
Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). Publisher’s version of record: https://doi.org/10.1126/sciadv.aaq1202