Optimized Formulation of a Thermostable Spray-Dried Virus-Like Particle Vaccine against Human Papillomavirus
Document Type
Article
Publication Date
5-2-2016
Abstract
© 2016 American Chemical Society. Existing vaccines against human papillomavirus (HPV) require continuous cold-chain storage. Previously, we developed a bacteriophage virus-like particle (VLP)-based vaccine for HPV infection, which elicits broadly neutralizing antibodies against diverse HPV types. Here, we formulated these VLPs into a thermostable dry powder using a multicomponent excipient system and by optimizing the spray-drying parameters using a half-factorial design approach. Dry-powder VLPs were stable after spray drying and after long-term storage at elevated temperatures. Immunization of mice with a single dose of reconstituted dry-powder VLPs that were stored at 37 °C for more than a year elicited high anti-L2 IgG antibody titers. Spray-dried thermostable, broadly protective L2 bacteriophage VLPs vaccine could be accessible to remote regions of the world (where ∼84% of cervical cancer patients reside) by eliminating the cold-chain requirement during transportation and storage.
Publication Title
Molecular Pharmaceutics
Recommended Citation
Saboo, S.,
Tumban, E.,
Peabody, J.,
Wafula, D.,
Peabody, D.,
Chackerian, B.,
&
Muttil, P.
(2016).
Optimized Formulation of a Thermostable Spray-Dried Virus-Like Particle Vaccine against Human Papillomavirus.
Molecular Pharmaceutics,
13(5), 1646-1655.
http://doi.org/10.1021/acs.molpharmaceut.6b00072
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/7818