Essential RNA-based technologies and their applications in plant functional genomics

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© 2015 Elsevier Ltd. Genome sequencing has not only extended our understanding of the blueprints of many plant species but has also revealed the secrets of coding and non-coding genes. We present here a brief introduction to and personal account of key RNA-based technologies, as well as their development and applications for functional genomics of plant coding and non-coding genes, with a focus on short tandem target mimics (STTMs), artificial microRNAs (amiRNAs), and CRISPR/Cas9. In addition, their use in multiplex technologies for the functional dissection of gene networks is discussed. RNA-based technologies are an essential toolbox for functional genomics in plants.We focus on technologies involving STTMs, amiRNAs/tasiRNAs, and sgRNA-guided genome editing.The article offers insights into how to improve RNA-based genome technologies, and their applications for functional discovery of coding and non-coding genes and their networks.

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Trends in Biotechnology