Essential RNA-based technologies and their applications in plant functional genomics
Document Type
Article
Publication Date
1-1-2016
Abstract
© 2015 Elsevier Ltd. Genome sequencing has not only extended our understanding of the blueprints of many plant species but has also revealed the secrets of coding and non-coding genes. We present here a brief introduction to and personal account of key RNA-based technologies, as well as their development and applications for functional genomics of plant coding and non-coding genes, with a focus on short tandem target mimics (STTMs), artificial microRNAs (amiRNAs), and CRISPR/Cas9. In addition, their use in multiplex technologies for the functional dissection of gene networks is discussed. RNA-based technologies are an essential toolbox for functional genomics in plants.We focus on technologies involving STTMs, amiRNAs/tasiRNAs, and sgRNA-guided genome editing.The article offers insights into how to improve RNA-based genome technologies, and their applications for functional discovery of coding and non-coding genes and their networks.
Publication Title
Trends in Biotechnology
Recommended Citation
Teotia, S.,
Singh, D.,
Tang, X.,
&
Tang, G.
(2016).
Essential RNA-based technologies and their applications in plant functional genomics.
Trends in Biotechnology,
34(2), 106-123.
http://doi.org/10.1016/j.tibtech.2015.12.001
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/7158