Determination of intracellular pH using sensitive, clickable fluorescent probes
Document Type
Article
Publication Date
4-1-2012
Department
Department of Chemistry
Abstract
We synthesized and evaluated a series of acidic fluorescent pH probes exhibiting robust pH dependence, high sensitivity and photostability, and excellent cell membrane permeability. Titration analyses indicated that probe 3 could increase its fluorescence intensity 800-fold between pH 8.0 and 4.1. Additionally, its pK a value is optimal for intracellular probing of acidic organelles. Fluorescent imaging of HepG2 and Hela cells further revealed that probe 3 demonstrates outstanding capacity for monitoring of intracellular [H +] levels. The easily accessible terminal alkyne/azido function groups of these probes offer the possibility of rapidly constructing sensor molecule libraries using 'click' chemistry.
Publication Title
Bioorganic and Medicinal Chemistry Letters
Recommended Citation
Yapici, N.,
Mandalapu, S.,
Chew, T.,
Khuon, S.,
&
Bi, L.
(2012).
Determination of intracellular pH using sensitive, clickable fluorescent probes.
Bioorganic and Medicinal Chemistry Letters,
22(7), 2440-2443.
http://doi.org/10.1016/j.bmcl.2012.02.021
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/6054