Document Type
Article
Publication Date
4-22-2019
Department
Department of Chemistry
Abstract
Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine
Publication Title
Molecules
Recommended Citation
Fang, M.,
Xia, S.,
Bi, J.,
Wigstrom, T.,
Valenzano, L.,
Wang, J.,
Tanasova, M.,
Luck, R.,
&
Liu, H.
(2019).
Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes..
Molecules,
24(8), 1-14.
http://doi.org/10.3390/molecules24081592
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/541
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Version
Publisher's PDF
Publisher's Statement
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Publisher’s version of record: https://doi.org/10.3390/molecules24081592