Effects of media components and environmental factors on shoot formation from protoplast-derived calli of Solanum tuberosum
Document Type
Article
Publication Date
11-1-1989
Department
Department of Biological Sciences
Abstract
The influence of several media components and environmental factors on shoot formation in protoplast-derived calli of Solanum tuberosum (a Rosamunda cross) were studied. Low sucrose concentration (3-15 mM) was beneficial for optimal shoot induction. Several concentrations of NO3- and NH4+ were suitable for shoot induction as long as the concentration of NO3- was about twice the concentration of NH4+ or higher. No stimulatory effect of glutamine, proline, putrescine, spermidine, spermine or adenine sulphate at 0.5 and 2 mM were found. White light promoted shoot induction compared with red and blue light or darkness. The intensity of light was shown to be a critical factor for good shoot induction. Lower light intensity (30 μE m-2 s-1) resulted in doubling of the number of calli producing shoots compared with higher (110 μE m-2 s-1) light intensity. A temperature of 20°C promoted shoot regeneration compared to 25°C. Based on these results improved conditions for regeneration of S. tuberosum are suggested, and shown to enhance shoot formation in five other genotypes tested.
Publication Title
Plant Cell, Tissue and Organ Culture
Recommended Citation
Lillo, C.
(1989).
Effects of media components and environmental factors on shoot formation from protoplast-derived calli of Solanum tuberosum.
Plant Cell, Tissue and Organ Culture,
19(2), 103-111.
http://doi.org/10.1007/BF00035810
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/4210