Knockdown of rice microRNA166 by short tandem target mimic (STTM)
Document Type
Article
Publication Date
1-1-2017
Abstract
© Springer Science+Business Media LLC 2017. Small RNAs, including microRNAs (miRNAs), are abundant in plants and play key roles in controlling plant development and physiology. miRNAs regulate the expression of the target genes involved in key plant processes. Due to functional redundancy among miRNA family members in plants, an ideal approach to silence the expression of all members simultaneously, for their functional characterization, is desirable. Target mimic (TM) was the first approach to achieve this goal. Short tandem target mimic (STTM) is a potent approach complementing TM for silencing miRNAs in plants. STTMs have been successfully used in dicots to block miRNA functions. Here, we describe in detail the protocol for designing STTM construct to block miRNA functions in rice. Such approach can be applied to silence miRNAs in other monocots as well.
Publication Title
Methods in Molecular Biology
Recommended Citation
Teotia, S.,
Zhang, D.,
&
Tang, G.
(2017).
Knockdown of rice microRNA166 by short tandem target mimic (STTM).
Methods in Molecular Biology,
1654, 337-349.
http://doi.org/10.1007/978-1-4939-7231-9_25
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/4053