Teratogenic effects of retinoic acid and dirmethylsulfoxide on embryonic chick wing and somite
Document Type
Article
Publication Date
12-1987
Department
Department of Biological Sciences
Abstract
In order to document the stage(s) at which the embryonic chick wing bud is sensitive to vitamin A teratogenesis and the kinds of defects produced by vitamin A insult to the embryonic chick wing, 1‐μg doses of retinoic acid (1 μl RA in 90% DMSO at a concentration of 1 μg/μl) were locally applied to the right wing bud of chick embryos at stages 17–23 (Hamburger and Hamilton: J. Morphol., 88:49‐92, '51), and the resulting limb skeleton anatomy was observed at 10 days of incubation. Local application of RA at stages 17–20 resulted in distal wing skeleton defects. There were significantly more wing skeleton defects among embryos treated at these stages with RA solution than among solvent (DMSO)‐treated control embryos and than among untreated control embryos. Wings of embryos treated with RA at stages 21–23 were always normal. Scapular and vertebral defects were seen at 10 days of incubation among embryos which had been treated prior to stage 21 with both the RA solution and the solvent control. Statistical analysis and histological data suggest that scapular and vertebral defects were caused by DMSO‐in‐duced damage to somites.
Publication Title
Teratology
Recommended Citation
Larsen, H.,
&
Janners, M.
(1987).
Teratogenic effects of retinoic acid and dirmethylsulfoxide on embryonic chick wing and somite.
Teratology,
36(3), 313-320.
http://doi.org/10.1002/tera.1420360307
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/3927
Publisher's Statement
Copyright © 1987 Wiley‐Liss, Inc., A Wiley Company. Publisher’s version of record: https://doi.org/10.1002/tera.1420360307