The DEAD-box helicase RCF1 plays roles in miRNA biogenesis and RNA splicing in Arabidopsis
Document Type
Article
Publication Date
7-6-2023
Department
Department of Biological Sciences
Abstract
RCF1 is a highly conserved DEAD-box RNA helicase found in yeast, plants and mammals. Studies about the functions of RCF1 in plants are limited. Here we uncovered the functions of RCF1 in Arabidopsis thaliana as a player in pri-miRNA processing and splicing, as well as in pre-mRNA splicing. A mutant with miRNA biogenesis defects was isolated and the defect was traced to a recessive point mutation in RCF1 (rcf1-4). We show that RCF1 promotes D-body formation and facilitates the interaction between pri-miRNAs and HYL1. Finally, we show that intron-containing pri-miRNAs and pre-mRNAs exhibit a global splicing defect in rcf1-4. Together, this work uncovers roles for RCF1 in miRNA biogenesis and RNA splicing in Arabidopsis.
Publication Title
The Plant journal : for cell and molecular biology
Recommended Citation
Xu, C.,
Zhang, Z.,
He, J.,
Bai, Y.,
Cui, J.,
Liu, L.,
Tang, J.,
Tang, G.,
Chen, X.,
&
Mo, B.
(2023).
The DEAD-box helicase RCF1 plays roles in miRNA biogenesis and RNA splicing in Arabidopsis.
The Plant journal : for cell and molecular biology,
116(1), 144-160.
http://doi.org/10.1111/tpj.16366
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/17354