Near-infrared mitochondria-targeted fluorescent probe with a large Stokes shift for rapid and sensitive detection of cysteine/homocysteine and its bioimaging application

Authors

Shixuan An, Zhejiang Normal University
Yanfei Lin, Jiaxing University
Jianbo Wang, Jiaxing University
Tianqing Ye, Jiaxing University
Yanyun Mao, Jiaxing University
Jian Zhang, Jiaxing University
Longhua Guo, Jiaxing University
Lei Li, Jiaxing University
Zhaosheng Qian, Zhejiang Normal University
Haiying Liu, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

1-1-2023

Department

Department of Chemistry

Abstract

In this work, tetrahydro-acridine salt hybrid coumarin dyes was designed and developed as a Near-infrared (NIR) fluorescent probe CM for the detection and fluorescent bioimaging of Cys/Hcy. The activated chlorine atom in probe facilitates more efficient nucleophilic substitution reaction, resulting in fast responsive time (3 min toward 3 eq. Cys) and high sensitivity toward Cys with detection limit of 46 nM. More importantly, probe displays “Turn-on” near-infrared fluorescence emission (Emmax: 674 nm) with Large Strokes shift (about 200 nm) toward Cys/Hcy. Furthermore, probe could be used as efficient tool for imaging and detection of Cys/Hcy in mitochondria of living cells and in vivo. This work innovatively proposed a new design strategy for NIR fluorescent probe platform for the detection and fluorescence imaging of bio-thiols.

Publication Title

Sensors and Actuators B: Chemical

Recommended Citation

An, S., Lin, Y., Wang, J., Ye, T., Mao, Y., Zhang, J., Guo, L., Li, L., Qian, Z., & Liu, H. (2023). Near-infrared mitochondria-targeted fluorescent probe with a large Stokes shift for rapid and sensitive detection of cysteine/homocysteine and its bioimaging application. Sensors and Actuators B: Chemical, 374. http://doi.org/10.1016/j.snb.2022.132799
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/16457