Near-infrared mitochondria-targeted fluorescent probe with a large Stokes shift for rapid and sensitive detection of cysteine/homocysteine and its bioimaging application
Document Type
Article
Publication Date
1-1-2023
Department
Department of Chemistry
Abstract
In this work, tetrahydro-acridine salt hybrid coumarin dyes was designed and developed as a Near-infrared (NIR) fluorescent probe CM for the detection and fluorescent bioimaging of Cys/Hcy. The activated chlorine atom in probe facilitates more efficient nucleophilic substitution reaction, resulting in fast responsive time (3 min toward 3 eq. Cys) and high sensitivity toward Cys with detection limit of 46 nM. More importantly, probe displays “Turn-on” near-infrared fluorescence emission (Emmax: 674 nm) with Large Strokes shift (about 200 nm) toward Cys/Hcy. Furthermore, probe could be used as efficient tool for imaging and detection of Cys/Hcy in mitochondria of living cells and in vivo. This work innovatively proposed a new design strategy for NIR fluorescent probe platform for the detection and fluorescence imaging of bio-thiols.
Publication Title
Sensors and Actuators B: Chemical
Recommended Citation
An, S.,
Lin, Y.,
Wang, J.,
Ye, T.,
Mao, Y.,
Zhang, J.,
Guo, L.,
Li, L.,
Qian, Z.,
&
Liu, H.
(2023).
Near-infrared mitochondria-targeted fluorescent probe with a large Stokes shift for rapid and sensitive detection of cysteine/homocysteine and its bioimaging application.
Sensors and Actuators B: Chemical,
374.
http://doi.org/10.1016/j.snb.2022.132799
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/16457