Document Type
Article
Publication Date
2-8-2021
Department
College of Forest Resources and Environmental Science
Abstract
Cellular functions are diversified through intricate transcription regulations, and an understanding gene regulation networks is essential to elucidating many developmental processes and environmental responses. Here, we employed the Transcriptional-Activator Like effectors (TALes), which represent a family of transcription factors that are synthesized by members of the γ-proteobacterium genus Xanthomonas and secreted to host cells for activation of targeted host genes. Through delivery by the maize pathogen, Xanthomonas vasicola pv. vasculorum, designer TALes (dTALes), which are synthetic TALes, were used to induce the expression of the maize gene glossy3 (gl3), a MYB transcription factor gene involved in the cuticular wax biosynthesis. RNA-Seq analysis of leaf samples identified 146 gl3 downstream genes. Eight of the nine known genes known to be involved in the cuticular wax biosynthesis were up-regulated by at least one dTALe. A top-down Gaussian graphical model predicted that 68 gl3 downstream genes were directly regulated by GL3. A chemically induced mutant of the gene Zm00001d017418 from the gl3 downstream gene, encoding aldehyde dehydrogenase, exhibited a typical glossy leaf phenotype and reduced epicuticular waxes. The bacterial protein delivery of artificial transcription factors, dTALes, proved to be a straightforward and powerful approach for the revelation of gene regulation in plants.
Recommended Citation
Zhao, M.,
Peng, Z.,
Qin, Y.,
Zhang, L.,
Tian, B.,
Chen, Y.,
Wei, H.,
&
et. al.
(2021).
Bacterium-Enabled Transient Gene Activation by Artificial Transcription Factor for Resolving Gene Regulation in Maize.
http://doi.org/10.1101/2021.02.05.429970
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15914
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 International License.
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Publisher's PDF
Publisher's Statement
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license. Publisher’s version of record: https://doi.org/10.1101/2021.02.05.429970