Graphene Oxide Functionalized Biosensor for Detection of Stress-Related Biomarkers

Authors

Erican Santiago, Michigan Technological UniversityFollow
Shailu Shree Poudyal, South Dakota State University
Sung Y. Shin, South Dakota State University
Hyeun Joong Yoon, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

1-12-2022

Department

Department of Biomedical Engineering

Abstract

A graphene oxide (GO)-based cortisol biosensor was developed to accurately detect corti-sol concentrations from sweat samples at point-of-care (POC) sites. A reference electrode, counter electrode, and working electrode make up the biosensor, and the working electrode was functional-ized using multiple layers consisting of GO and antibodies, including Protein A, IgG, and anti-Cab. Sweat samples contact the anti-Cab antibodies to transport electrons to the electrode, resulting in an electrochemical current response. The sensor was tested at each additional functionalization layer and at cortisol concentrations between 0.1 and 150 ng/mL to determine how the current response differed. A potentiostat galvanostat device was used to measure and quantify the electrochemical response in the GO-based biosensor. In both tests, the electrochemical responses were reduced in magnitude with the addition of antibody layers and with increased cortisol concentrations. The proposed cortisol biosensor has increased accuracy with each additional functionalization layer, and the proposed device has the capability to accurately measure cortisol concentrations for diagnostic purposes.

Publisher's Statement

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Publisher’s version of record: https://doi.org/10.3390/s22020558

Publication Title

Sensors

Recommended Citation

Santiago, E., Poudyal, S., Shin, S., & Yoon, H. J. (2022). Graphene Oxide Functionalized Biosensor for Detection of Stress-Related Biomarkers. Sensors, 22(2). http://doi.org/10.3390/s22020558
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15684

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF