Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays
Document Type
Article
Publication Date
1-1-2022
Department
Department of Biomedical Engineering
Abstract
Micropillar arrays are one of the tools that quantify the mechanical forces exerted by endothelial cells and any other adherent cell types. This chapter describes the fabrication and usage of the micropillars that reports spatial distribution of traction forces by endothelial cells. The fabrication begins with making a “master” pillar substrate using photolithography, followed by softlithography of the master with polydimethylsiloxane. The pillars can then be printed with an extracellular matrix protein in specific shape using stamp-off technique. Lastly, imaging of the micropillars with cells and the analysis of the traction force using Matlab-based software are described.
Publication Title
Methods in Molecular Biology
Recommended Citation
Chandurkar, M.,
&
Han, S.
(2022).
Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays.
Methods in Molecular Biology,
2375, 229-245.
http://doi.org/10.1007/978-1-0716-1708-3_19
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15459