Black Dots: Microcontact-Printed, Reference-Free Traction Force Microscopy
Document Type
Article
Publication Date
8-3-2021
Department
Department of Biomedical Engineering
Abstract
Measuring the traction forces produced by cells provides insight into their behavior and physiological function. Here, we developed a technique (dubbed ‘black dots’) that microcontact prints a fluorescent micropattern onto a flexible substrate to measure cellular traction forces without constraining cell shape or needing to detach the cells. To demonstrate our technique, we assessed human platelets, which can generate a large range of forces within a population. We find platelets that exert more force have more spread area, are more circular, and have more uniformly distributed F-actin filaments. As a result of the high yield of data obtainable by this technique, we were able to evaluate multivariate mixed effects models with interaction terms and conduct a clustering analysis to identify clusters within our data. These statistical techniques demonstrated a complex relationship between spread area, circularity, F-actin dispersion, and platelet force, including cooperative effects that significantly associate with platelet traction forces.
Publication Title
bioRxiv
Recommended Citation
Beussman, K. M.,
Mollica, M. Y.,
Leonard, A.,
Miles, J.,
Hocter, J.,
Song, Z.,
Han, S. J.,
&
et. al.
(2021).
Black Dots: Microcontact-Printed, Reference-Free Traction Force Microscopy.
bioRxiv.
http://doi.org/10.1101/2021.08.02.454500
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15431
Version
Preprint
Publisher's Statement
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. © 2021. Publisher’s version of record: https://doi.org/10.1101/2021.08.02.454500