Rna in situ hybridization for detecting gene expression patterns in the abdomens and wings of drosophila species

Authors

Mujeeb Olushola Shittu, Michigan Technological UniversityFollow
Tessa Steenwinkel, Michigan Technological UniversityFollow
William Dion, University of Pittsburgh
Nathan Ostlund, Michigan Technological UniversityFollow
Komal Raja, Baylor College of Medicine
Thomas Werner, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

3-10-2021

Department

Department of Biological Sciences

Abstract

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

Publisher's Statement

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Publisher’s version of record: https://doi.org/10.3390/MPS4010020

Publication Title

Methods and Protocols

Recommended Citation

Shittu, M. O., Steenwinkel, T., Dion, W., Ostlund, N., Raja, K., & Werner, T. (2021). Rna in situ hybridization for detecting gene expression patterns in the abdomens and wings of drosophila species. Methods and Protocols, 4(1). http://doi.org/10.3390/MPS4010020
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/14850

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF