Continuous purification of an enveloped and non-enveloped viral particle using an aqueous two-phase system
Document Type
Article
Publication Date
8-15-2021
Department
Department of Chemical Engineering; Department of Mechanical Engineering-Engineering Mechanics
Abstract
Meeting the increasing demand for vaccines throughout the world is key to decrease the spread of infectious diseases. The switch to a fully continuous vaccine manufacturing process would increase productivity and the supply of crucial vaccines. To aid in this switch, we have developed a novel, continuous downstream purification technique based on an aqueous two-phase system (ATPS). The system has the potential to be used as a platform system for viral product purification. A 12 kDa poly(ethylene glycol) (PEG) and trisodium citrate ATPS was able to purify porcine parvovirus (PPV) and human immunodeficiency virus type-1 group antigen virus-like particles (HIV VLPs) from cell supernatant. PPV was recovered in the PEG-rich phase at 90 ± 16% with a DNA removal of 96 ± 3% and ≥89% protein removal. The system was also able to recover 99 ± 2% of HIV VLPs in the PEG-rich phase with a 73 ± 1% DNA removal and high protein removal shown by SDS-PAGE. Continuous ATPS recovered virus at the same amount as batch recovery. This demonstrates that continuous ATPS can be scaled up and runrun continuously without a loss in purity or recovery. Mixing and settling time both played an important role in developing a continuous ATPS for viral particles.
Publication Title
Separation and Purification Technology
Recommended Citation
Turpeinen, D. G.,
Joshi, P. U.,
Kriz, S.,
Kaur, S.,
Nold, N.,
O'Hagan, D.,
Nikam, S.,
Masoud, H.,
&
Heldt, C. L.
(2021).
Continuous purification of an enveloped and non-enveloped viral particle using an aqueous two-phase system.
Separation and Purification Technology,
269.
http://doi.org/10.1016/j.seppur.2021.118753
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/14768