Hydroxyl Radical Generation through the Fenton-like Reaction of Hematin- And Catechol-Functionalized Microgels

Authors

Pegah Kord Forooshani, Michigan Technological UniversityFollow
Rattapol Pinnaratip, Michigan Technological UniversityFollow
Elizabeth Polega, Michigan Technological UniversityFollow
Ariana Tyo, Michigan Technological UniversityFollow
Eric Pearson, Michigan Technological University
Bo Liu, Michigan Technological University
Tinu Ololade Folayan, Michigan Technological UniversityFollow
Lei Pan, Michigan Technological UniversityFollow
Rupak Rajachar, Michigan Technological UniversityFollow
Caryn L. Heldt, Michigan Technological UniversityFollow
Bruce P. Lee, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

10-13-2020

Department

Department of Chemical Engineering; Department of Biomedical Engineering

Abstract

Hydroxyl radical (•OH) is a potent reactive oxygen species with the ability to degrade hazardous organic compounds, kill bacteria, and inactivate viruses. However, an off-the-shelf, portable, and easily activated biomaterial for generating •OH does not exist. Here, microgels were functionalized with catechol, an adhesive moiety found in mussel adhesive proteins, and hematin (HEM), a hydroxylated Fe3+ ion-containing porphyrin derivative. When the microgel was hydrated in an aqueous solution with physiological pH, molecular oxygen in the solution oxidized catechol to generate H2O2, which was further converted to •OH by HEM. The generated •OH was able to degrade organic dyes, including orange II and malachite green. Additionally, the generated •OH was antimicrobial against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus epidermidis) bacteria with the initial concentration of 106 to 107 cfu/mL. These microgels also reduced the infectivity of a nonenveloped porcine parvovirus and an enveloped bovine viral diarrhea virus by 3.5 and 4.5 log reduction values, respectively (99.97-99.997% reduction in infectivity). These microgels were also functionalized with positively charged [2-(methacryloyloxy)ethyl] trimethylammonium chloride, which significantly enhanced the antibacterial and antiviral activities through electrostatic interaction between the negatively charged pathogens and the microgel. These microgels can potentially serve as a lightweight and portable source of disinfectant for an on-demand generation of •OH with a wide range of applications.

Publisher's Statement

Copyright © 2020 American Chemical Society. Publisher’s version of record: https://doi.org/10.1021/acs.chemmater.0c01551

Publication Title

Chemistry of Materials

Recommended Citation

Forooshani, P., Pinnaratip, R., Polega, E., Tyo, A., Pearson, E., Liu, B., Folayan, T., Pan, L., Rajachar, R., Heldt, C. L., & Lee, B. (2020). Hydroxyl Radical Generation through the Fenton-like Reaction of Hematin- And Catechol-Functionalized Microgels. Chemistry of Materials, 32(19), 8182-8194. http://doi.org/10.1021/acs.chemmater.0c01551
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/14375