Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using illumina sequencing
Document Type
Article
Publication Date
1-1-2017
Abstract
© 2017, Editura Silvica. All rights reserved. Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.
Publication Title
Annals of Forest Research
Recommended Citation
Wu, Y.,
Zhang, R.,
Staton, M.,
Schlarbaum, S.,
Coggeshall, M.,
Romero-Severson, J.,
Carlson, J.,
Zembower, N.,
Liang, H.,
Xu, Y.,
Drautz-Moses, D.,
Schuster, S.,
&
Gailing, O.
(2017).
Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using illumina sequencing.
Annals of Forest Research,
60(2), 343-350.
http://doi.org/10.15287/afr.2017.819
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/13513