The transgenic poplar as an efficient bioreactor system for the production of xylanase
Document Type
Article
Publication Date
6-29-2012
Abstract
Plants are attractive expression systems for largescale, low-cost production of high-value proteins. The xylanase 2 gene (Xyn2), encoding an endo-β-1,4-xylanase from Trichoderma reesei, was cloned and expressed in Escherichia coli and the poplar (Populus spp.). The optimal temperature and pH of the recombinant xylanase were 50 °C and 5.0 respectively when expressed in E. coli. The purpose of this study was to produce recombinant xylanase in poplar. The Xyn2 gene was transferred into poplars by Agrobacterium-mediated transformation. The transgenic status and transgene expression of the transformed poplar were confirmed by polymerase chain reaction (PCR) genotyping and reverse transcription (RT)-PCR analysis. The poplar-expressed xylanase was biologically active, with an expression level of up to 14.4% of total leaf soluble protein. In the leaves, the average xylanase content was 1.016mg per g of leaf fresh weight in the transgenic poplar. We found that the poplar might make possible the large-scale production of commercially important recombinant proteins.
Publication Title
Bioscience, Biotechnology and Biochemistry
Recommended Citation
Kim, S.,
Kim, Y.,
Lee, Y.,
Choi, I.,
Joshi, C.,
Lee, K.,
&
Bae, H.
(2012).
The transgenic poplar as an efficient bioreactor system for the production of xylanase.
Bioscience, Biotechnology and Biochemistry,
76(6), 1140-1145.
http://doi.org/10.1271/bbb.110981
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/13172