Document Type
Article
Publication Date
6-2018
Department
Department of Biomedical Engineering
Abstract
Human mesenchymal stem cell (hMSC) sheets hold great potential in engineering three-dimensional (3D) completely biological tissues for diverse applications. Conventional cell sheet culturing methods employing thermoresponsive surfaces are cost ineffective, and rely heavily on available facilities. In this study, a cost-effective method of layer-by-layer grafting was utilized for covalently binding a homogenous collagen I layer on a commonly used polydimethylsiloxane (PDMS) substrate surface in order to improve its cell adhesion as well as the uniformity of the resulting hMSC cell sheet. Results showed that a homogenous collagen I layer was obtained via this grafting method, which improved hMSC adhesion and attachment through reliable collagen I binding sites. By utilizing this low-cost method, a uniform hMSC sheet was generated. This technology potentially allows for mass production of hMSC sheets to fulfill the demand of thick hMSC constructs for tissue engineering and biomanufacturing applications.
Publication Title
Bioactive Materials
Recommended Citation
Qian, Z.,
Ross, D.,
Jia, W.,
Xing, Q.,
&
Zhao, F.
(2018).
Bioactive polydimethylsiloxane surface for optimal human mesenchymal stem cell sheet culture.
Bioactive Materials,
3(2), 167-173.
http://doi.org/10.1016/j.bioactmat.2018.01.005
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/1315
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Version
Publisher's PDF
Publisher's Statement
©2018 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/). Publisher’s version of record: https://doi.org/10.1016/j.bioactmat.2018.01.005