Ratiometric fluorescent and colorimetric BODIPY-based sensor for zinc ions in solution and living cells

Authors

Shuai Xia, Michigan Technological UniversityFollow
Jinjin Shen, Jiaxing University
Jianbo Wang, Jiaxing University
Hailong Wang, Jiaxing University
Mingxi Fang, Michigan Technological UniversityFollow
Hongwei Zhou, Jiaxing University
Marina Tanasova, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

11-22-2017

Department

Department of Chemistry

Abstract

Zinc ion detection in solution and living cells through colorimetric and ratiometric fluorescence with the novel Boron-dipyrromethene (BODIPY) based probe (BNDP) is described. The targeted modification of the established Bodipy fluorophore to trap zinc ions into the tetra-coordinating complex enables quantitative analysis of zinc ion concentrations detectable visually and through fluorescence due to the apparent color change between unbound and bound detection probe species. The equivalent-molar BNDP: Zn²⁺ complexation, evident through the Job’s plot, ¹H NMR, and ESI–MS occurs with low nano-molar affinity, and induces significant changes in spectroscopic properties of BNDP, resulting in 27 nm and 40 nm red-shift in absorption and emission spectra, respectively. The complex is stable at physiological pH, allowing monitoring and quantifying levels of Zn²⁺ concentration change in living cells, and detecting intracellular Zn²⁺ released from intracellular metalloproteins.

Publication Title

Sensors and Actuators B: Chemical

Recommended Citation

Xia, S., Shen, J., Wang, J., Wang, H., Fang, M., Zhou, H., & Tanasova, M. (2017). Ratiometric fluorescent and colorimetric BODIPY-based sensor for zinc ions in solution and living cells. Sensors and Actuators B: Chemical, 258, 1279-1286. http://doi.org/10.1016/j.snb.2017.11.129
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/1280