Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl.
Document Type
Article
Publication Date
11-1-2011
Abstract
For the first time in sessile oak [Quercus petraea (Matt.) Liebl.], the isolation and characterisation of a full-length dehydrin gene and its promoter region, as well as its allelic variation in natural populations, is reported. Dehydrins (Dhn) are stress-related genes important for the survival of perennial plants in a seasonal climate. A full-length dehydrin gene (Dhn3) was characterised at the nucleotide level and the protein structure was modelled. Additionally, the allelic variation was analysed in five natural populations of Quercus petraea (Matt.) Liebl. sampled along an altitudinal gradient in the French Pyrenees. The analysed sequences contain typical domains of the K n class of dehydrins in the coding region. Also, the 5′untranslated region (promoter) of the gene was amplified, which shows typical motifs essential for drought- and cold-responsive gene expression. Single nucleotide substitutions and indels (insertions/deletions) within the coding region determine large biochemical differences at the protein level. However, only low levels of genetic differentiation between populations from different altitudes were detectable. © 2011 German Botanical Society and The Royal Botanical Society of the Netherlands.
Publication Title
Plant Biology
Recommended Citation
Vornam, B.,
Gailing, O.,
Derory, J.,
Plomion, C.,
Kremer, A.,
&
Finkeldey, R.
(2011).
Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl..
Plant Biology,
13(6), 881-887.
http://doi.org/10.1111/j.1438-8677.2011.00446.x
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/11329