Date of Award

2019

Document Type

Campus Access Dissertation

Degree Name

Doctor of Philosophy in Chemistry (PhD)

Administrative Home Department

Department of Chemistry

Advisor 1

Lanrong Bi

Committee Member 1

Rudy Luck

Committee Member 2

Sarah Green

Committee Member 3

Qinghui Chen

Committee Member 4

Jingfang Ju

Abstract

My thesis is focused on the development of fluorescent probes for biosensing and bioimaging within specific organelles. My main research efforts are mainly focused on the design, synthesis and biological applications of these new molecular probes. These new fluorescent probes I developed can be manipulated through the chemical modifications for binding to specific organelles capable of reporting localized bioinformation. Compared to the currently commercially available organelle-specific fluorescent stains, the advantages of the newly synthesized fluorescent probes include low cytotoxicity, high photostability, and long fluorescence lifetimes. These features are crucial for long-time tracking study of biological processes.

Research on fluorescent probes with both analyte responsiveness and organelle targetability is a new and emerging area that has attracted increasing attention over the past few years. Because of their high sensitivity, specificity and fast response, these novel fluorescent probes have been proven to be useful tools for facilitating biomedical research. I have further extended the diversity by developing organelle-specific responsive probes capable of detecting changes in biomolecular levels and the microenvironment. My future research efforts give more considerations of the “low-concern” organelles, such as the Golgi apparatus, the endoplasmic reticulum, and ribosomes. Considering the tiny sizes of subcellular organelles, we anticipate that better visualization of the cellular events within specific organelles will rely on super-resolution optical microscopy with nanoscopic-scale resolution.

Available for download on Saturday, April 18, 2020

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