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Date of Award
Campus Access Master's Thesis
Master of Science in Environmental Engineering (MS)
Administrative Home Department
Department of Civil and Environmental Engineering
Committee Member 1
Tetrachloroethene (PCE) is a common groundwater contaminant. Several bacterial strains have the ability to respire PCE and/or its daughter products through dehalorespiration, and can be used to completely detoxify aqueous PCE. PCE contamination is often present as a dense non-aqueous phase liquid (DNAPL), which serves as a long-term contaminant source that slowly dissolves into the groundwater. This study examined the potential for dehalorespiration to bioenhance dissolution in an intermediate-scale flow cell (ISFC) containing a PCE DNAPL pool. The overall project goal was to examine how the interactions between two dehalorespirers, Desulfuromonas michiganensis BB1 and Dehalococcoides mccartyi 195, impacted PCE dissolution and detoxification. Abiotic experiments indicated that PCE dissolution was affected by porous medium heterogeneities that altered the hydrodynamic conditions. Introduction of the two dehalorespirers initially bioenhanced the PCE dissolution rate by 2-3X, with Dsm. michiganensis BB1 dominating. Microbial growth later caused bioclogging, which increased flow adjacent to the DNAPL-aqueous interface, and further enhanced PCE dissolution.
Klemm, Sara, "Bioenhanced Dissolution of a Tetrachloroethene (PCE) Pool in a Sand Tank Aquifer System", Campus Access Master's Thesis, Michigan Technological University, 2016.