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Date of Award
Campus Access Master's Report
Master of Science in Biological Sciences (MS)
Administrative Home Department
Department of Biological Sciences
Committee Member 1
Chandrashekhar P. Joshi
Committee Member 2
Type 2 Diabetes is a metabolic disorder affecting millions of people worldwide. The long-term effects include cardiovascular complications, neurological damage, kidney failure, cirrhosis, and death. Altered miRNA levels have shown to have tremendous amount of effect on pancreatic β-cells which has led to increase in metabolic stress like glucotoxicity, lipotoxicity and oxidative stress. Gamma-Glutamyl Transferase has been studied for its role in human function since the 1960s and is now employed in clinical laboratories as a biomarker for liver damage. Recently, many studies have proved GGT to be a marker for oxidative stress and correlated it to understanding the adverse outcomes of several diseases. In this study, we designed and performed experiments to establish that absence of miR-483 in pancreatic β-cells induced oxidative stress. miR-483 knockout mice pancreatic islets were subjected to many lipotoxic, and cytokine/ inflammatory stress which induced oxidative stress to mimic Type 2 Diabetes condition. We found GGT1 levels increased significantly in the miR-483 knockdown mice when compared to the control mice. In conclusion, we can deduce that miR-483 deficiency causes oxidative stress in the cells.
Mehta, Nirali, "Micro-RNA 483 deficiency increases oxidative stress in Pancreatic Beta-cells", Campus Access Master's Report, Michigan Technological University, 2023.