Off-campus Michigan Tech users: To download campus access theses or dissertations, please use the following button to log in with your Michigan Tech ID and password: log in to proxy server

Non-Michigan Tech users: Please talk to your librarian about requesting this thesis or dissertation through interlibrary loan.

Date of Award


Document Type

Campus Access Master's Report

Degree Name

Master of Science in Biological Sciences (MS)

Administrative Home Department

Department of Biological Sciences

Advisor 1

Xiaoqing Tang

Committee Member 1

Chandrashekhar P. Joshi

Committee Member 2

Zhiying Shan


Type 2 Diabetes is a metabolic disorder affecting millions of people worldwide. The long-term effects include cardiovascular complications, neurological damage, kidney failure, cirrhosis, and death. Altered miRNA levels have shown to have tremendous amount of effect on pancreatic β-cells which has led to increase in metabolic stress like glucotoxicity, lipotoxicity and oxidative stress. Gamma-Glutamyl Transferase has been studied for its role in human function since the 1960s and is now employed in clinical laboratories as a biomarker for liver damage. Recently, many studies have proved GGT to be a marker for oxidative stress and correlated it to understanding the adverse outcomes of several diseases. In this study, we designed and performed experiments to establish that absence of miR-483 in pancreatic β-cells induced oxidative stress. miR-483 knockout mice pancreatic islets were subjected to many lipotoxic, and cytokine/ inflammatory stress which induced oxidative stress to mimic Type 2 Diabetes condition. We found GGT1 levels increased significantly in the miR-483 knockdown mice when compared to the control mice. In conclusion, we can deduce that miR-483 deficiency causes oxidative stress in the cells.