Denaturing reversed-phase HPLC using a mobile phase containing urea for oligodeoxynucleotide analysis

Authors

Suntara Fueangfung, Michigan Technological University
Yinan Yuan, Michigan Technological University
Shiyue Fang, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

6-27-2014

Abstract

Denaturing reversed-phase (RP) high performance liquid chromatography (HPLC) is usually achieved by elevating column temperature. In this article, an alternative method involving using a mobile phase that contains urea and performing HPLC at room temperature is described. The efficacy of the new method was demonstrated by analyzing a 61-mer oligodeoxynucleotide (ODN) and double-stranded (ds) ODNs. The multiple peaks of the 61-mer ODN under normal conditions merged into one under the denaturing conditions. The broad single peaks of dsODNs under normal conditions were split into two sharp peaks.

Publisher's Statement

Copyright Taylor and Francis Group, LLC. Publisher’s version of record: https://doi.org/10.1080/15257770.2014.891740

Publication Title

Nucleosides, Nucleotides and Nucleic Acids

Recommended Citation

Fueangfung, S., Yuan, Y., & Fang, S. (2014). Denaturing reversed-phase HPLC using a mobile phase containing urea for oligodeoxynucleotide analysis. Nucleosides, Nucleotides and Nucleic Acids, 33(7), 481-488. http://doi.org/10.1080/15257770.2014.891740
Retrieved from: https://digitalcommons.mtu.edu/chemistry-fp/129