Comparison of high-throughput sequencing for phage display peptide screening in two commercially available platforms

Document Type

Article

Publication Date

4-19-2019

Abstract

Phage display is a widely used technique to screen peptide sequences for interaction with target biomolecules, such as proteins and cells. Traditional protocols screen for only a limited fraction of a candidate pool due to the cost and time limitation of Sanger sequencing. Recent developments in high-throughput sequencing (HTS) technologies enable researchers to assess millions of biomolecule sequences. In this study, eluted DNA pooled from a phage display screening were sequenced by two types of HTS methodologies; sequence by synthesis and a nanopore-based techniques. While both methods resulted in the identification of several candidate peptide motifs determined to be interacting with the target proteins, the sequence by synthesis method provide a higher yield of valid reads for data analyses. Input library preparation protocol for the nanopore sequencer needs further modification to achieve effective data collection.

Publisher's Statement

© Springer Nature B.V. 2019. Publisher's version of record: https://doi.org/10.1007/s10989-019-09858-8

Publication Title

International Journal of Peptide Research and Therapeutics

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