Title
Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectors
Document Type
Article
Publication Date
8-15-2019
Abstract
Adeno-associated virus (AAV) is a strong candidate for single-gene mutation gene therapy. AAV comes in several serotypes that target different organs in the body. The current purification methods for AAV vectors often rely on serotype dependent affinity chromatography. However, it is desired to create a platform for AAV purification that mirrors the evolution of antibody platform processes. To do this, any serotype dependent steps need to be removed from the process. The harvest and initial capture steps that can satisfy all of the needs of a platform AAV process is the use of low pH and Triton in the harvest, followed by filtration and cation exchange chromatography (CEX) for initial capture. The low pH hydrolyses and removes the host cell DNA, a difficult contaminate to remove. CEX then provides a concentration and capture step. The only step that remains is to determine the polishing and final formulation. This harvest strategy provides a serotype independent purification that removes both host cell DNA and host cell proteins and is friendly to scale-up for future AAV processes.
Publication Title
Journal of Chromatography B
Recommended Citation
Heldt, C. L.
(2019).
Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectors.
Journal of Chromatography B,
1124, 173-179.
http://doi.org/10.1016/j.jchromb.2019.06.011
Retrieved from: https://digitalcommons.mtu.edu/chemical-fp/17
Publisher's Statement
© 2019 Elsevier B.V. All rights reserved. Publisher’s version of record: https://doi.org/10.1016/j.jchromb.2019.06.011