Title
S-nitroso-N-acetylpenicillamine (SNAP) derivatization of peptide primary amines to create inducible nitric oxide donor biomaterials
Document Type
Article
Publication Date
7-24-2013
Abstract
An S-nitroso-N-acetylpenicillamine (SNAP) derivatization approach was used to modify existing free primary amines found in fibrin (a natural protein-based biomaterial) to generate a controlled nitric oxide (NO) releasing scaffold material. The duration of the derivatization reaction affects the NO release kinetics, the induction of controlled NO-release, hydrophobicity, swelling behavior, elastic moduli, rheometric character, and degradation behavior. These properties were quantified to determine changes in fibrin hydrogels following covalent attachment of SNAP. NO-releasing materials exhibited minimal cytotoxicity when cultured with fibroblasts or osteoblasts. Cells maintained viability and proliferative character on derivatized materials as demonstrated by Live/Dead cell staining and counting. In addition, SNAP-derivatized hydrogels exhibited an antimicrobial character indicative of NO-releasing materials. SNAP derivatization of natural polymeric biomaterials containing free primary amines offers a means to generate inducible NO-releasing biomaterials for use as an antimicrobial and regenerative support for tissue engineering.
Publication Title
Applied Materials and Interfaces
Recommended Citation
VanWagner, M.,
Rhadigan, J.,
Lancina, M.,
Lebovsky, A.,
Romanowicz, G.,
Holmes, H.,
Brunette, M. A.,
Snyder, K. L.,
Bostwick, M.,
Lee, B. P.,
Frost, M. C.,
&
Rajachar, R.
(2013).
S-nitroso-N-acetylpenicillamine (SNAP) derivatization of peptide primary amines to create inducible nitric oxide donor biomaterials.
Applied Materials and Interfaces,
5(17), 8430-8439.
http://doi.org/10.1021/am4017945
Retrieved from: https://digitalcommons.mtu.edu/biomedical-fp/16
Publisher's Statement
© 2013 American Chemical Society. Publisher's version of record: http://dx.doi.org/10.1021/am4017945